Grapevine is one of the most important crops grown worldwide and has become essential model plant for studying multiple interactions in perennial woody species. Pathogenic viruses represent serious biotic stress to a grapevine for many disturbances which could result in reduced yield, grape quality and shortened lifespan. On the other hand, root-beneficial microorganisms such as arbuscular mycorrhizal fungi (AMF) have a potential to alleviate the stress of biotic and abiotic origin. The background of biological processes in the grapevine, in the light of tripartite interaction is completely under-investigated despite being predominantly present in agroecosystems worldwide.

To provide novel insight in complex interactions between grapevine virus and AMF, the main goal of INTER-OMICS is to investigate core changes in transcriptome profiles by high-throughput RNA-sequencing approach. The differentially expressed genes (DEGs) in grapevine will be identified and associated with agronomic grapevine performances. Changes in AMF transcriptome profile will also be studied. Grapevine metabolome profiling will reveal whether and to what extent DEG changes are reflected in the grapevine metabolome. By examining DEGs and metabolome in leaves and roots, better understanding of processes of grapevine-virus-AMF interactions in upper- and under-ground plant organs and their synthesis into overall plant behaviour will be reached. INTER-OMICS aims to deepen the understanding of molecular mechanisms beyond manifested changes and reveal some unknown aspects of grapevine-virus-AMF interactions. Gained results could present pioneer base for development of natural metabolite-based strategies in agricultural practice for enhancing grapevine resistance or mitigating viral damage.